Viewers last saw his character marry nurse Trixie Franklin.
Call the Midwife actor Olly Rix, who plays Matthew Aylward, is set to leave the BBC One show, as per The Sun.
The series's latest season, which concluded in February, saw Matthew marry senior nurse Trixie Franklin (Helen George) following their romance across the seasons.
An insider claimed: "The news of Olly's departure has left the cast and crew stunned and upset. They all thought he was a mainstay on the series after his prominent wedding scenes to Helen last season."
A spokesperson for the drama declined to comment when approached by RadioTimes.com.
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Earlier on the year, Rix revealed how he made the filming for Matthew and Trixie's big day in Call the Midwife extra special.
"I did everything I could to make sure that I didn't see Helen before she arrived on set," he explained to Ranvir Singh during an appearance on Lorraine.
"I had no idea what the dress was gonna look like, and I said to the guys dropping us down there, 'Please make sure we don't cross paths, I don't want to see her until I see her walking down the aisle.'
"Amazingly, it worked, and it was a bit of a heart-stopping moment."
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Viewers can expect a return to Poplar in the upcoming Christmas special in December, with the 13th season set to air next year.
As revealed in the Radio Times magazine, the upcoming episodes are set to welcome some new faces to the show.
Student midwives and roommates Joyce and Rosalind, played by Renee Bailey and Natalie Quarry, will be joining the cast.
All 12 seasons of Call the Midwife are available to stream on BBC iPlayer. Check out more of our Drama coverage or visit our TV Guide and Streaming Guide to see what's on tonight.
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Katelyn Mensah is the Entertainment and Factual Writer for Radio Times, covering all major entertainment programmes, reality TV shows and the latest hard-hitting documentaries. She previously worked at The Tab, with a focus on reality TV and showbiz news and has obtained a BA (Hons) in Journalism.
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